Types of culture media pdf

The act of organism culturing into the media is called inoculation or streaking. - The common method to obtain pure culture (isolated colony) is dry dilution that should be done under septic conditions to prevent growth of contaminants. Types of media and their functions 1- Basal media: allows growth of most non pathogenic bacteria. E.g Types of Culture Media - Free download as Powerpoint Presentation (.ppt / .pptx), PDF File (.pdf) or view presentation slides online. Scribd is the world's largest social reading and publishing site. Save Save Types of Culture Media For Later. 0% 0% found this document useful, Mark this document as useful. 0% 0% found this document not Cultures derived from mushrooms can be grown on Potato Dextrose Agar, Potato Sucrose Agar and Malt Extract Agar. Potato Dextrose Yeast Extract Agar (PDYA) also is a recommended Pl P 421 General Mycology medium for growing cultures of Agaricus, Pleurotus, Lentinus, Stropharia, Flammulina, and some of the Psilocybespecies. Plant Tissue Culture Media 31 culture media [12]. Nicotinic acid is used at a concentration range 0.1-5 mg.l-1 and pyridoxine is used at 0.1-10 mg.l-1. Other vitamins such as biotin, folic acid, ascorbic acid, pantothenic acid, tocopherol (vitamin E), riboflavin, p-amino-benzoic acid are used in some cell culture media however, they are not 2.1.1. Types of serum Serum can derive from different animals. Current serum used in tissue culture is cattle serum. Human serum, horse serum is used for some specific cells. Cattle serum has several advantages as used in cell culture: adequate resource, mature preparation technique, long application time. Synthetic or defined media • specially prepared media from pure chemical substances for research purpose and composition of every component is well known • eg: peptone water - 1% peptone + 0.5% NaCl in water. 13. Special media • Enriched media • Selective media • Differential media • Transport media • Anaerobic media. Types of media Types of media Enriched media Selective media Differential media contains specific growth factors needed by fastidious bacteria to support their growth. Examples -blood agar -chocolate agar used to select (isolate) specific group of bacteria these can distinguish among morphologically and biochemically related Culture Media. Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms. Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them. Growth media are primarily of two types; one In this article we will discuss about Culture Media:- 1. Meaning of Culture Media 2. Components of Culture Media 3. Culture Procedures. Meaning of Culture Media: Media requirements depend on the type of microorganism being used in the fermentation process, but the basic requirements remain the same, which includes: (a) Source of energy (b) Water, […] The culture media (nutrients) consist of chemicals which support the growth of culture or microorganisms. Microbes can use the nutrients of culture media as their food is necessary for cultivating them in vitro. Types of Culture Media: The first medium prepared was meat-infusion broth. media or colonies. 3. Assay media: Media of prescribed composition are used for the assay of vitamins, amino acids, antibiotics etc. 4. Enumeration media: Specific kinds of media are used fo