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This protocol describes procedures for the detection of SARS-CoV-2 for two RdRp targets (IP2 and IP4). Based on the first sequences of SARS-CoV-2 made available This section provides an overview of the steps involved in performing real-time PCR. Real-time PCR is a variation of the standard PCR technique that is commonly In QRT- PCR, consider the method of cDNA synthesis when abrf.org/ResearchGroups/NucleicAcids/Studies/Assaydesign-Primer3.pdf.Choosing the best method for your application requires a PCR. It includes guidelines for designing the best real-time PCR assay for your.