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Many immunoassay methods use enzymes to develop chromogenic response, and are termed enzyme immunoassays. Assays that generate a chromogenic response are Introduction. • Immunoassays are a diverse group of analytical techniques which are found throughout clinical, biopharmaceutical, and environmental fields. In immunoassay testing, the analyte may be either an antibody, or an antigen. Summary of Immunoassay Techniques. Immunoassays utilize one or more select When a lateral flow immunoassay is run, the test sample is added to a sample application pad at the end of the strip. The sample then migrates to the conjugate